Anti-HLA-DR APC

Anti-HLA-DR APC

• Background: HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.
• Specifications: The antibody MEM-12 recognizes a common extracellular epitope on human HLA-DR which is dependent on the association of alpha and beta chains. DR is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC dendritic cells, B lymphocytes, monocytes, macrophages).
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: thymocyte membrane
• Target Antigen: HLA-DR
• Isotype: IgG1
• Clone: MEM-12
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: APC
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Horejsi V, Nemec M, Angelisova P, Kristofova H, Gorga JC, Hilgert I: Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens. Tissue Antigens. 1986 Nov28(5):288-97., URL: https://pubmed.ncbi.nlm.nih.gov/2435017/,*Sochorová K, Budínský V, Rožková D, Tobiášová Z, Dusilová-Sulková S, Spíšek R, Bartůňková J: Paricalcitol (19-nor-1,25-dihydroxyvitamin D2) and calcitriol (1,25-dihydroxyvitamin D3) exert potent immunomodulatory effects on dendritic cells and inhibit induction of antigen-specific T cells. Clin Immunol. 2009 Oct133(1):69-77., URL: https://pubmed.ncbi.nlm.nih.gov/19660988/,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.