Anti-Hu CD147 APC

CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6) also known as EMMPRIN (extracellular matrix metalloproteinase inducer) or TCSF (tumour cell-derived collagenase-stimulatory factor) is an ubiquitously expressed cell surface protein with multiple glycosylated forms. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumour cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.

Human

Protein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2-and CH3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimer) .

CD147

MEM-M6/1

FC (QC tested)

APC

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Staffler G, Szekeres A, Schütz GJ, Säemann MD, Prager E, Zeyda M, Drbal K, Zlabinger GJ, Stulnig TM, Stockinger H: Selective inhibition of T cell activation via CD147 through novel modulation of lipid rafts. J Immunol. 2003 Aug 15;171 (4) :1707-14., URL: https://www.ncbi.nlm.nih.gov/pubmed/12902469 , *Baba M, Inoue M, Itoh K, Nishizawa Y: Blocking CD147 induces cell death in cancer cells through impairment of glycolytic energy metabolism. Biochem Biophys Res Commun. 2008 Sep 12;374 (1) :111-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/18616931 , *Cho JY, Fox DA, Horejsi V, Sagawa K, Skubitz KM, Katz DR, Chain B: The functional interactions between CD98, beta1-integrins, and CD147 in the induction of U937 homotypic aggregation. Blood. 2001 Jul 15;98 (2) :374-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/11435306 , *Pistol G, Matache C, Calugaru A, Stavaru C, Tanaseanu S, Ionescu R, Dumitrache S, Stefanescu M: Roles of CD147 on T lymphocytes activation and MMP-9 secretion in systemic lupus erythematosus. J Cell Mol Med. 2007 Mar-Apr;11 (2) :339-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/17488482 , *Ohradanova-Repic A, Machacek C, Charvet C, Lager F, Le Roux D, Platzer R, Leksa V, Mitulovic G, Burkard TR, Zlabinger GJ, Fischer MB, Feuillet V, Renault G, Blüml S, Benko M, Suchanek M, Huppa JB, Matsuyama T, Cavaco-Paulo A, Bismuth G, Stockinger H: Extracellular Purine Metabolism Is the Switchboard of Immunosuppressive Macrophages and a Novel Target to Treat Diseases With Macrophage Imbalances. Front Immunol. 2018 Apr 27;9:852., URL: https://www.ncbi.nlm.nih.gov/pubmed/29780382 , *Schatzlmaier P, Supper V, Göschl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 22;8 (395) :rs11, URL: http://www.ncbi.nlm.nih.gov/pubmed/26396269 , *Koch C, Staffler G, Huttinger R, Hilgert I, Prager E, Cerny J, Steinlein P, Majdic O, Horejsi V, Stockinger H: T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density. Int Immunol. 1999 May;11 (5) :777-86., URL: https://pubmed.ncbi.nlm.nih.gov/10330283/

*Kirk P, Wilson MC, Heddle C, Brown MH, Barclay AN, Halestrap AP: CD147 is tightly associated with lactate transporters MCT1 and MCT4 and facilitates their cell surface expression. EMBO J. 2000 Aug 1;19 (15) :3896-904., URL: https://www.ncbi.nlm.nih.gov/pubmed/10921872?ordin, *Wilson MC, Meredith D, Fox JE, Manoharan C, Davies AJ, Halestrap AP: Basigin (CD147) is the target for organomercurial inhibition of monocarboxylate transporter isoforms 1 and 4: the ancillary protein for the insensitive MCT2 is EMBIGIN (gp70) . J Biol Chem. 2005 Jul 22;280 (29) :27213-21., URL: https://www.ncbi.nlm.nih.gov/pubmed/15917240?ordin, *Xu D, Hemler ME: Metabolic activation-related CD147-CD98 complex. Mol Cell Proteomics. 2005 Aug;4 (8) :1061-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/15901826?ordin, *Ruiz S, Castro-Castro A, Bustelo XR: CD147 Inhibits the Nuclear Factor of Activated T-cells by Impairing Vav1 and Rac1 Downstream Signaling. J Biol Chem. 2008 Feb 29;283 (9) :5554-66., URL: https://www.ncbi.nlm.nih.gov/pubmed/18160397?ordin, *Iacono KT, Brown AL, Greene MI, Saouaf SJ: CD147 immunoglobulin superfamily receptor function and role in pathology. Exp Mol Pathol. 2007 Dec;83 (3) :283-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/17945211?ordin, *Melchior A, Denys A, Deligny A, Mazurier J, Allain F: Cyclophilin B induces integrin-mediated cell adhesion by a mechanism involving CD98-dependent activation of protein kinase C-delta and p44/42 mitogen-activated protein kinases. Exp Cell Res. 2008 Feb 1;314 (3) :616-28., URL: https://www.ncbi.nlm.nih.gov/pubmed/18054915?ordin, *Schmidt R, Bültmann A, Fischel S, Gillitzer A, Cullen P, Walch A, Jost P, Ungerer M, Tolley ND, Lindemann S, Gawaz M, Schömig A, May AE. Extracellular matrix metalloproteinase inducer (CD147) is a novel receptor on platelets, activates platelets, and augments nuclear factor kappaB-dependent inflammation in monocytes. Circ Res. 2008 Feb 15;102 (3) :302-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/18048771?ordin

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The antibody MEM-M6/1 recognizes an extracellular epitope in the N-terminal Ig domain (D1) of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes. The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.

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