Anti-Hu CD137 APC

CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.

Human, Non-human primates

Recombinant human CD137 ectodomain

CD137

4B4-1

FC (QC tested)

APC

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Bellarosa D, Bressan A, Bigioni M, Parlani M, Maggi CA, Binaschi M: SAHA/Vorinostat induces the expression of the CD137 receptor/ligand system and enhances apoptosis mediated by soluble CD137 receptor in a human breast cancer cell line. Int J Oncol. 2012 Oct;41 (4) :1486-94., URL: http://www.ncbi.nlm.nih.gov/pubmed/22797667 , *Fernández Do Porto DA, Jurado JO, Pasquinelli V, Alvarez IB, Aspera RH, Musella RM, García VE: CD137 differentially regulates innate and adaptive immunity against Mycobacterium tuberculosis. Immunol Cell Biol. 2012 Apr;90 (4) :449-56., URL: http://www.ncbi.nlm.nih.gov/pubmed/21747409 , *Yi L, Zhao Y, Wang X, Dai M, Hellström KE, Hellström I, Zhang H: Human and mouse CD137 have predominantly different binding CRDs to their respective ligands. PLoS One. 2014 Jan 21;9 (1) :e86337., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=yi+l+zhao+y+wang+x , *Chan SL, Voskens CJ, Lin W, Schindler DG, Azimzadeh A, Wang LX, Taylor RJ, Strome SE, Schulze DH: Epitope mapping of a chimeric CD137 mAb: a necessary step for assessing the biologic relevance of non-human primate models. J Mol Recognit. 2009 May-Jun;22 (3) :242-9. doi: 10.1002/jmr.937., URL: http://www.ncbi.nlm.nih.gov/pubmed/19177494, *Kienzle G, von Kempis J: CD137 (ILA/4-1BB), expressed by primary human monocytes, induces monocyte activation and apoptosis of B lymphocytes. Int Immunol. 2000 Jan;12 (1) :73-82., URL: https://pubmed.ncbi.nlm.nih.gov/10607752/, *Salih HR, Kosowski SG, Haluska VF, Starling GC, Loo DT, Lee F, Aruffo AA, Trail PA, Kiener PA: Constitutive expression of functional 4-1BB (CD137) ligand on carcinoma cells. J Immunol. 2000 Sep 1;165 (5) :2903-10. , URL: https://pubmed.ncbi.nlm.nih.gov/10946324/

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The mouse monoclonal antibody 4B4-1 recognizes an extracellular conformational epitope on CD137, an approximately 40 kDa type I transmembrane protein of the TNFR family expressed mainly on activated T cells.

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