Anti-Ms NK1.1 Purified Low Endotoxin

Anti-Ms NK1.1 Purified Low Endotoxin

• Background: NK1.1 / CD161bc (also known as NKRP1, Ly55c, or Ly59) is a cell surface antigen expressed on NK cells and NK-T cells of certain mouse strains, which is being used for identification or antibody-mediated depletion of these cells in the respective strains. This antigen participates in NK cell activation, including production of interferon gamma, and release of cytotoxic granules.
• Specifications: The mouse monoclonal antibody PK136 recognizes an extracellular epitope of NK1.1 antigen (NK cell marker) expressed by some mouse strains (e.g. C57BL/6, NZB, CE, C58, Ma/My), whereas other strains (e.g. BALB/c, AKR, CBA, C3H) do not express this antigen. This antibody detects a conformational epitope on Nkrp1c and Nkrp1b gene products.
• Certification: RUO
• Host: Mouse
• Species Reactivity: Mouse
• Immunogen: NK1-positive murine splenic and bone marrow cells
• Target Antigen: NK1.1
• Isotype: IgG2a κ
• Clone: PK136
• Applications: Functional application: In vivo depletion of NK cells and NK-T cells. Flow cytometry: Recommended dilution: 1-4 µg/mL
• Validated Applications: FC, IP, IHC-F, Functional assays
• Concentration: 1 mg/mL
• Format: Purified Low Endotoxin
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• References & Citations: *Carlyle JR, Mesci A, Ljutic B, Belanger S, Tai LH, Rousselle E, Troke AD, Proteau MF, Makrigiannis AP: Molecular and genetic basis for strain-dependent NK1.1 alloreactivity of mouse NK cells. J Immunol. 2006 Jun 15176(12):7511-24., URL: http://www.ncbi.nlm.nih.gov/pubmed/16751398 ,*GeurtsvanKessel CH, Bergen IM, Muskens F, Boon L, Hoogsteden HC, Osterhaus AD, Rimmelzwaan GF, Lambrecht BN: Both conventional and interferon killer dendritic cells have antigen-presenting capacity during influenza virus infection. PLoS One. 2009 Sep 284(9):e7187. , URL: http://www.ncbi.nlm.nih.gov/pubmed/19784375 ,*Símová J, Bubeník J, Bieblová J, Jandlová T: The role of NK1.1+ cells in the protection against MHC class I+ HPV16-associated tumours. Folia Biol (Praha). 200450(6):200-2., URL: http://www.ncbi.nlm.nih.gov/pubmed/15709715 ,*Carroll JL, Nielsen LL, Pruett SB, Mathis JM: The role of natural killer cells in adenovirus-mediated p53 gene therapy. Mol Cancer Ther. 2001 Nov1(1):49-60., URL: http://www.ncbi.nlm.nih.gov/pubmed/12467238 ,*Joncker NT, Shifrin N, Delebecque F, Raulet DH: Mature natural killer cells reset their responsiveness when exposed to an altered MHC environment. J Exp Med. 2010 Sep 27207(10):2065-72., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=mature+natural+killer+cells+reset ,*Wang M, Ellison CA, Gartner JG, HayGlass KT: Natural killer cell depletion fails to influence initial CD4 T cell commitment in vivo in exogenous antigen-stimulated cytokine and antibody responses. J Immunol. 1998 Feb 1160(3):1098-105., URL: http://www.ncbi.nlm.nih.gov/pubmed/9570522 ,*Takahashi S, Kawamura T, Kanda Y, Taniguchi T, Nishizawa T, Iiai T, Hatakeyama K, Abo T: Activation of CD1d-independent NK1.1+ T cells in the large intestine by Lactobacilli. Immunol Lett. 2006 Jan 15102(1):74-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/16107279 ,*Fang M, Lanier LL, Sigal LJ: A role for NKG2D in NK cell-mediated resistance to poxvirus disease. PLoS Pathog. 2008 Feb 84(2):e30., URL: http://www.ncbi.nlm.nih.gov/pubmed/18266471 ,*Kulesza J, Hoser G, Wasilewska D, Kawiak J: NK cell depletion and recovery in SCID mice treated with anti-NK1.1 antibody. Folia Histochem Cytobiol. 200644(2):93-6., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=kulesza+j+hoser+g ,*Laffont S, Seillet C, Ortaldo J, Coudert JD, Guéry JC: Natural killer cells recruited into lymph nodes inhibit alloreactive T-cell activation through perforin-mediated killing of donor allogeneic dendritic cells. Blood. 2008 Aug 1112(3):661-71, URL: http://www.ncbi.nlm.nih.gov/pubmed/18505782,
• Storage Conditions: Store at 2-8°C. Do not freeze.