Anti-Hu CD86 Purified Low Endotoxin

Anti-Hu CD86 Purified Low Endotoxin

• Background: CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
• Specifications: The mouse monoclonal antibody BU63 reacts with an extracellular epitope of CD86 (B7-2), a 70 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: B-lymphoblastoid cell line ARH 77
• Target Antigen: CD86
• Isotype: IgG1
• Clone: BU63
• Applications: Functional application: The antibody BU63 blocks mixed lymphocyte reaction (mLR) and binding of soluble CTLA-4 (CD152)-mulg fusion protein to CD86 (B7-2). Flow cytometry: Recommended dilution: 5 μg/mL.
• Validated Applications: FC, IP, WB, IHC-F, Functional assays
• Concentration: 1 mg/mL
• Format: Purified Low Endotoxin
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• References & Citations: *Zhan H, Towler HM, Calder VL: The immunomodulatory role of human conjunctival epithelial cells. Invest Ophthalmol Vis Sci. 2003 Sep44(9):3906-10., URL: https://www.ncbi.nlm.nih.gov/pubmed/12939308,*Kolar GR, Mehta D, Pelayo R, Capra JD: A novel human B cell subpopulation representing the initial germinal center population to express AID. Blood. 2007 Mar 15109(6):2545-52., URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC18521,*Hovden AO, Karlsen M, Jonsson R, Aarstad HJ, Appel S: Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses. BMC Immunol. 2011 Jan 512:2., URL: http://www.ncbi.nlm.nih.gov/pubmed/21208424 ,*Chan A, Baird M, Mercer AA, Fleming SB: Maturation and function of human dendritic cells are inhibited by orf virus-encoded interleukin-10. J Gen Virol. 2006 Nov87(Pt 11):3177-81., URL: http://www.ncbi.nlm.nih.gov/pubmed?term=chan%20a%20baird%20m ,*Ohradanova-Repic A, Machacek C, Charvet C, Lager F, Le Roux D, Platzer R, Leksa V, Mitulovic G, Burkard TR, Zlabinger GJ, Fischer MB, Feuillet V, Renault G, Blüml S, Benko M, Suchanek M, Huppa JB, Matsuyama T, Cavaco-Paulo A, Bismuth G, Stockinger H: Extracellular Purine Metabolism Is the Switchboard of Immunosuppressive Macrophages and a Novel Target to Treat Diseases With Macrophage Imbalances. Front Immunol. 2018 Apr 279:852., URL: https://www.ncbi.nlm.nih.gov/pubmed/29780382 ,*Engel P, Gribben JG, Freeman GJ, Zhou LJ, Nozawa Y, Abe M, Nadler LM, Wakasa H, Tedder TF: The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen. Blood. 1994 Sep 184(5):1402-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/7520767?ordina,*Caux C, Vanbervliet B, Massacrier C, Azuma M, Okumura K, Lanier LL, Banchereau J: B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells. J Exp Med. 1994 Nov 1180(5):1841-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/7525840?ordina,*Mauri D, Wyss-Coray T, Gallati H, Pichler WJ: Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules. J Immunol. 1995 Jul 1155(1):118-27., URL: https://www.ncbi.nlm.nih.gov/pubmed/7541409?ordina,*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., URL: ,*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., URL: ,*Giguère JF, Bounou S, Paquette JS, Madrenas J, Tremblay MJ: Insertion of host-derived costimulatory molecules CD80 (B7.1) and CD86 (B7.2) into human immunodeficiency virus type 1 affects the virus life cycle. J Virol. 2004 Jun78(12):6222-32., URL: https://www.ncbi.nlm.nih.gov/pubmed/15163715?ordin,
• Other References: *Eri R, Kodumudi KN, Summerlin DJ, Srinivasan M: Suppression of colon inflammation by CD80 blockade: Evaluation in two murine models of inflammatory bowel disease. Inflamm Bowel Dis. 2008 Jan 9, URL: https://www.ncbi.nlm.nih.gov/pubmed/18186109?ordin,
• Storage Conditions: Store at 2-8°C. Do not freeze.