Anti-Hu CD112 Purified

• Description:

  CD112, also known as nectin-2, is a transmembrane glycoprotein involved in organization of adherens junctions. It also serves as a target molecule for entry of certain strains of herpes simplex virus (HSV) and pseudorabies virus (PRV) . It is homologous to CD155, which serves as a target molecule for polio virus. CD112 seems to play a role in neural tube formation, with N-cadherin. Inside the cell, CD112 is connected with actin cytoskeleton through afadin. Variations in the CD112 gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

• Certification:

  RUO

• Reactivity:

  Human

• Immunogen:

  NIH/3T3 cells transfected with human Nectin-2

• Target Antigen:

  CD112

• Clone:

  R2.525

• Applications:

  FC (QC tested), IP, IHC-F

• Concentration:

  1 mg/mL

• Format:

  Purified

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Carlsten M, Björkström NK, Norell H, Bryceson Y, van Hall T, Baumann BC, Hanson M, Schedvins K, Kiessling R, Ljunggren HG, Malmberg KJ: DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells. Cancer Res. 2007 Feb 1;67 (3) :1317-25., URL: http://www.ncbi.nlm.nih.gov/pubmed/17283169 , *Hou S, Ge K, Zheng X, Wei H, Sun R, Tian Z: CD226 protein is involved in immune synapse formation and triggers Natural Killer (NK) cell activation via its first extracellular domain. J Biol Chem. 2014 Mar 7;289 (10) :6969-77., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=hou+s+ge+k+zheng+x Nielsen N Ø dum N Ursø B Lanier LL Spee P: Cytotoxicity of CD56 (bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D LFA-1 and TRAIL and dampened via, *Enqvist M, Nilsonne G, Hammarfjord O, Wallin RP, Björkström NK, Björnstedt M, Hjerpe A, Ljunggren HG, Dobra K, Malmberg KJ, Carlsten M: Selenite induces posttranscriptional blockade of HLA-E expression and sensitizes tumor cells to CD94/NKG2A-positive NK cells. J Immunol. 2011 Oct 1;187 (7) :3546-54., URL: https://pubmed.ncbi.nlm.nih.gov/21890659/

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Specificity:

  The mouse monoclonal antibody R2.525 recognizes an extracellular epitope on CD112, a type I transmembrane glycoprotein expressed by myelomonocytic and megakaryocytic cells, and by CD34+ hematopoietic progenitors.

• Applications Notes:

  Flow cytometry: Recommended dilution: 1-4 μg/ml.

• Isotype:

  Mouse IgG1 kappa