Anti-LIME1 Purified

Anti-LIME1 Purified

• Background: LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
• Specifications: The Polyclonal antibody reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Rabbit
• Species Reactivity: Human
• Negative Species: Mouse
• Immunogen: Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.
• Target Antigen: LIME1
• Clonality: Polyclonal
• Clone: PAb (410)
• Validated Applications: WB, IHC-P
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V. LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17198(10):1453-62., URL: ,
• Other References: *Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug4(8):603-16., URL: https://pubmed.ncbi.nlm.nih.gov/15286727/,*Simeoni L, Smida M, Posevitz V, Schraven B, Lindquist JA: Right time, right place: the organization of membrane proximal signaling. Semin Immunol. 2005 Feb17(1):35-49., URL: https://pubmed.ncbi.nlm.nih.gov/15582487/,
• Storage Conditions: Store at 2-8°C. Do not freeze.