Anti-Hu CD13 Purified

Anti-Hu CD13 Purified

• Background: CD13 (aminopeptidase N, APN) is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.
• Specifications: The antibody WM15 recognises an extracellular epitope of human CD13 cell surface glycoprotein, a 150 kDa molecule expressed on granulocytes, endothelial cells, epithelial cells and myeloid progenitors.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human, Non-human primates
• Immunogen: Human AML cells
• Target Antigen: CD13
• Isotype: IgG1
• Clone: WM15
• Applications: Flow cytometry: Recommended dilution: 1-4 μg/mL.
• Validated Applications: FC, IP, IHC-F
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Favaloro EJ, Browning T, Facey D: CD13 (GP150 aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated. Exp Hematol. 1993 Dec21(13):1695-701. , URL: https://www.ncbi.nlm.nih.gov/pubmed/7902291?dopt=A,*McCormack E, Mujic M, Osdal T, Bruserud O, Gjertsen BT: Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia. Blood. 2013 Feb 14121(7):e34-42. doi: 10.1182/blood-2012-05-429555., URL: https://www.ncbi.nlm.nih.gov/pubmed/23243270,*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., URL: ,*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989)., URL: ,*Leukocyte Typing III., McMichael A.J. et al. (Eds.), Oxford University Press (1987)., URL: ,*Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Musgrove E: Myeloid progenitor surface antigen identified by monoclonal antibody. Br J Haematol. 1985 Sep61(1):11-20., URL: https://www.ncbi.nlm.nih.gov/pubmed/4052321?dopt=A,*Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Berndt MC, Musgrove E: Human myeloid differentiation antigens identified by monoclonal antibodies: expression on leukemic cells. Pathology. 1985 Jul17(3):392-9. , URL: https://www.ncbi.nlm.nih.gov/pubmed/3865147?dopt=A,
• Other References: *Tokuhara T, Hattori N, Ishida H, Hirai T, Higashiyama M, Kodama K, Miyake M. Clinical significance of aminopeptidase N in non-small cell lung cancer. Clin Cancer Res. 2006 Jul 112(13):3971-8., URL: https://pubmed.ncbi.nlm.nih.gov/16818694/,*Petrovic N, Schacke W, Gahagan JR, O'Conor CA, Winnicka B, Conway RE, Mina-Osorio P, Shapiro LH. CD13/APN regulates endothelial invasion and filopodia formation. Blood. 2007 Jul 1110(1):142-50., URL: https://pubmed.ncbi.nlm.nih.gov/17363739/,*Terauchi M, Kajiyama H, Shibata K, Ino K, Nawa A, Mizutani S, Kikkawa F. Inhibition of APN/CD13 leads to suppressed progressive potential in ovarian carcinoma cells. BMC Cancer. 2007 Jul 277:140., URL: https://pubmed.ncbi.nlm.nih.gov/17655775/,
• Storage Conditions: Store at 2-8°C. Do not freeze.