Anti-Hu CD16 Purified

• Description:

  CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.

• Certification:

  RUO

• Reactivity:

  Non-human primates, Human, Pig

• Immunogen:

  Human granulocytes

• Target Antigen:

  CD16

• Clone:

  MEM-168

• Applications:

  FC (QC tested)

• Concentration:

  1 mg/mL

• Format:

  Purified

• Buffer:

  Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

• References & Citations:

  *Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19 (5) :675-84., URL: http://www.ncbi.nlm.nih.gov/pubmed/17446208?itool=

• Other References:

  *Gessner JE, Grussenmeyer T, Kolanus W, Schmidt RE: The human low affinity immunoglobulin G Fc receptor III-A and III-B genes. Molecular characterization of the promoter regions. J Biol Chem. 1995 Jan 20;270 (3) :1350-61., URL: https://pubmed.ncbi.nlm.nih.gov/7836402/, *Kocher M, Siegel ME, Edberg JC, Kimberly RP: Cross-linking of Fc gamma receptor IIa and Fc gamma receptor IIIb induces different proadhesive phenotypes on human neutrophils. J Immunol. 1997 Oct 15;159 (8) :3940-8., URL: https://pubmed.ncbi.nlm.nih.gov/9378982/, *Arase N, Arase H, Hirano S, Yokosuka T, Sakurai D, Saito T: IgE-mediated activation of NK cells through Fc gamma RIII. J Immunol. 2003 Mar 15;170 (6) :3054-8., URL: https://pubmed.ncbi.nlm.nih.gov/12626560/

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Specificity:

  The antibody MEM-168 reacts with an extracellular epitope of CD16 antigen, a low affinity receptor for aggregated IgG (FcgammaRIII antigen) . CD16 exists in two different isoforms: CD16a (FcgammaRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB; 48 kDa; mainly expressed on neutrophils) .

• Applications Notes:

  Flow cytometry: Recommended dilution: 1-4 μg/ml.

• Isotype:

  Mouse IgM