Anti-Hu CD33 Purified

Anti-Hu CD33 Purified

• Background: CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.
• Specifications: The antibody HIM3-4 reacts with an extracellular epitope of CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Non-human primates, Human
• Immunogen: NFMY-9s human cell line
• Target Antigen: CD33
• Isotype: IgG1
• Clone: HIM3-4
• Applications: Flow cytometry: Recommended dilution: 1-5 μg/mL.
• Validated Applications: FC
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *McCormack E, Mujic M, Osdal T, Bruserud O, Gjertsen BT: Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia. Blood. 2013 Feb 14121(7):e34-42. doi: 10.1182/blood-2012-05-429555., URL: http://www.ncbi.nlm.nih.gov/pubmed/23243270,
• Other References: *Walter RB, Häusermann P, Raden BW, Teckchandani AM, Kamikura DM, Bernstein ID, Cooper JA: Phosphorylated ITIMs Enable Ubiquitylation of an Inhibitory Cell Surface Receptor. Traffic. 2007 Dec 18, URL: https://www.ncbi.nlm.nih.gov/pubmed/18062779?ordin,*Orr SJ, Morgan NM, Elliott J, Burrows JF, Scott CJ, McVicar DW, Johnston JA: CD33 responses are blocked by SOCS3 through accelerated proteasomal-mediated turnover. Blood. 2007 Feb 1109(3):1061-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/17008544?ordin,*Ulyanova T, Blasioli J, Woodford-Thomas TA, Thomas ML: The sialoadhesin CD33 is a myeloid-specific inhibitory receptor. Eur J Immunol. 1999 Nov29(11):3440-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/10556798?ordin,
• Storage Conditions: Store at 2-8°C. Do not freeze.