Anti-Hu CD44 Purified

CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.

Pig, Human, Dog

COS-7 cells (African Green Monkey) .

CD44

MEM-263

FC (QC tested), IP, WB, IHC-P (reported)

1 mg/mL

Purified

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Vigetti D, Viola M, Karousou E, Rizzi M, Moretto P, Genasetti A, Clerici M, Hascall VC, De Luca G, Passi A: Hyaluronan-CD44-ERK1/2 regulate human aortic smooth muscle cell motility during aging. J Biol Chem. 2007 Dec 12, URL: https://www.ncbi.nlm.nih.gov/pubmed/18077444?ordin, *Hollingsworth JW, Li Z, Brass DM, Garantziotis S, Timberlake SH, Kim A, Hossain I, Savani RC, Schwartz DA. CD44 regulates macrophage recruitment to the lung in lipopolysaccharide-induced airway disease. Am J Respir Cell Mol Biol. 2007 Aug;37 (2) :248-53., URL: https://www.ncbi.nlm.nih.gov/pubmed/17446529?ordin, *Liu J, Bi G, Wen P, Yang W, Ren X, Tang T, Xie C, Dong W, Jiang G. Down-regulation of CD44 contributes to the differentiation of HL-60 cells induced by ATRA or HMBA. Cell Mol Immunol. 2007 Feb;4 (1) :59-63., URL: https://www.ncbi.nlm.nih.gov/pubmed/17349212?ordin, *Subramaniam V, Gardner H, Jothy S: Soluble CD44 secretion contributes to the acquisition of aggressive tumor phenotype in human colon cancer cells. Exp Mol Pathol. 2007 Dec;83 (3) :341-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/17945213?ordin, *Subramaniam V, Vincent IR, Gardner H, Chan E, Dhamko H, Jothy S: CD44 regulates cell migration in human colon cancer cells via Lyn kinase and AKT phosphorylation. Exp Mol Pathol. 2007 Oct;83 (2) :207-15., URL: https://www.ncbi.nlm.nih.gov/pubmed/17599831?ordin

Store at 2-8°C. Do not freeze.

The antibody MEM-263 reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.) ; it is negative on platelets and hepatocytes.

Flow cytometry: Recommended dilution: 4 μg/ml. Western blotting: Recommended dilution: 2 μg/ml, 60 min on vertical incubator; positive control: Kg-1a human acute leukemia cell lysate, JURKAT human leukemia T-cell lysate, non-reducing conditions, 6% separating gel. Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml; positive tissue: uterus, myometrium.