Anti-Hu IgE Purified

• Description:

  Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.

• Certification:

  RUO

• Reactivity:

  Human

• Immunogen:

  Purified human IgE.

• Target Antigen:

  IgE

• Clone:

  4G7.325

• Applications:

  ELISA, FC

• Concentration:

  1 mg/mL

• Format:

  Purified

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Ovsyannikova IG, Vailes LD, Li Y, Heymann PW, Chapman MD: Monoclonal antibodies to group II Dermatophagoides spp. allergens: murine immune response, epitope analysis, and development of a two-site ELISA. J Allergy Clin Immunol. 1994 Sep;94 (3 Pt 1) :537-46., URL: https://pubmed.ncbi.nlm.nih.gov/7521892/

• Other References:

  *Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh) . 1975;194:77-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/47690?ordinalp, *Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21 (6) :667-71., URL: https://www.ncbi.nlm.nih.gov/pubmed/1122610?ordina, *Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39 (2) :129-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/6966908?ordina, *Gould HJ, Beavil RL, Vercelli D: IgE isotype determination: epsilon-germline gene transcription, DNA recombination and B-cell differentiation. Br Med Bull. 2000;56 (4) :908-24., URL: https://www.ncbi.nlm.nih.gov/pubmed/11359628?ordin, *Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152 (2) :323-32., URL: https://www.ncbi.nlm.nih.gov/pubmed/8258141?ordina

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Specificity:

  The mouse monoclonal antibody 4G7.325 reacts with human IgE; it recognizes an epitope different from the ones recognized by BE5 and 4H10 antibodies to IgE. The epitope is located within the amino acids 103-115 (WSDYNFDYSSSEE) .

• Applications Notes:

  Flow cytrometry: Recommended dilution: 1-4 μg/ml.

• Isotype:

  Mouse IgG