Anti-Hu CD25 Purified

• Description:

  CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R) . Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.

• Certification:

  RUO

• Reactivity:

  Human

• Immunogen:

  PHA-activated peripheral blood leucocytes

• Target Antigen:

  CD25

• Clone:

  MEM-140

• Applications:

  FC (QC tested), IP

• Concentration:

  1 mg/mL

• Format:

  Purified

• Buffer:

  Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

• Other References:

  *Sotlar K, Horny HP, Simonitsch I, Krokowski M, Aichberger KJ, Mayerhofer M, Printz D, Fritsch G, Valent P: CD25 indicates the neoplastic phenotype of mast cells: a novel immunohistochemical marker for the diagnosis of systemic mastocytosis (SM) in routinely processed bone marrow biopsy specimens. Am J Surg Pathol. 2004 Oct;28 (10) :1319-25., URL: https://www.ncbi.nlm.nih.gov/pubmed/15371947?ordin, *Morris JC, Waldmann TA: Advances in interleukin 2 receptor targeted treatment. Ann Rheum Dis. 2000 Nov;59 Suppl 1:i109-14., URL: https://www.ncbi.nlm.nih.gov/pubmed/11053100?ordin, *Scheibenbogen C, Keilholz U, Richter M, Andreesen R, Hunstein W: The interleukin-2 receptor in human monocytes and macrophages: regulation of expression and release of the alpha and beta chains (p55 and p75) . Res Immunol. 1992 Jan;143 (1) :33-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/1565844?ordina, *Lai KN, Leung JC, Lai FM: Soluble interleukin 2 receptor release, interleukin 2 production, and interleukin 2 receptor expression in activated T-lymphocytes in vitro. Pathology. 1991 Jul;23 (3) :224-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/1838146?ordina

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Specificity:

  +

• Applications Notes:

  +

• Isotype:

  Mouse IgM