Anti-Hu CD151 Purified

Anti-Hu CD151 Purified

• Background: CD151, also known as PETA-3 (platelet-endothelial tetraspan antigen), is a four-pass transmembrane glycoprotein with short cytoplasmic N- and C-termini. CD151 is expressed mainly in platelets and megakaryocytes, immature hematopoietic cells, activated T cells, in endothelium, muscle cells, and epithelial cells. It associates with CD9, CD181, and integrin complexes alpha 3 / beta 1 (CD49c / CD29), alpha 5 / beta 1 (CD49e / CD29), and alpha 6 / beta 4 (CD49f / CD104). CD151 appears to be involved in cell adhesion and migration, including metastasis.
• Specifications: The mouse monoclonal antibody CD151 recognizes an extracellular epitope of CD151 (also known as PETA-3), a 29 kDa transmembrane protein of tetraspanin family, expressed in many cell types.
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Human epidermoid carcinoma cell line Hep-3
• Target Antigen: CD151
• Isotype: Igg1 κ
• Clone: 50-6
• Applications: Flow cytometry: Recommended dilution: 2-4 μg/mL.
• Validated Applications: IHC-F, WB, FC, ICC
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Sadej R, Grudowska A, Turczyk L, Kordek R, Romanska HM: CD151 in cancer progression and metastasis: a complex scenario. Lab Invest. 2014 Jan94(1):41-51., URL: http://www.ncbi.nlm.nih.gov/pubmed/24247563,*Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, Chasis JA, Anstee DJ: Tetraspanins CD81 and CD82 facilitate 94549461-mediated adhesion of human erythroblasts to vascular cell adhesion molecule-1. PLoS One. 2013 May 218(5):e62654., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=spring+fa+griffiths+re Testa JE Brooks PC Lin JM Quigley JP: Eukaryotic expression cloning with an antimetastatic monoclonal antibody identifies a tetraspanin (PETA-3/CD151) as an effector of human tumor cell ,
• Storage Conditions: Store at 2-8°C. Do not freeze.