Anti-Cytokeratins Purified

• Description:

  Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

• Certification:

  RUO

• Reactivity:

  Mammalian

• Immunogen:

  Keratin-enriched preparation from human epidermoid carcinoma cell line A431.

• Target Antigen:

  Cytokeratins

• Clone:

  C-11

• Applications:

  FC-IC, WB, IP, IHC-P, ICC

• Concentration:

  1 mg/mL

• Format:

  Purified

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Hamakawa H, Sumida T, Tanioka H, Sogawa K, Yamada T: Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis. Res Commun Mol Pathol Pharmacol. 1998 Aug;101 (2) :115-26., URL: https://pubmed.ncbi.nlm.nih.gov/9821208/, *Broekema M, Harmsen MC, Koerts JA, Petersen AH, van Luyn MJ, Navis G, Popa ER: Determinants of tubular bone marrow-derived cell engraftment after renal ischemia/reperfusion in rats. Kidney Int. 2005 Dec;68 (6) :2572-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/16316332?dopt=, *Vojtesek B, Stasková Z, Nenutil R, Lauerová L, Kovarík J, Rejthar A, Bártková J, Bártek J: Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha) . 1989;35 (6) :373-82., URL: https://www.ncbi.nlm.nih.gov/pubmed/2483834, *Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl. 1988;3:50-5. , URL: https://www.ncbi.nlm.nih.gov/pubmed/2463228?dopt=A, *Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164 (3) :215-24., URL: https://www.ncbi.nlm.nih.gov/pubmed/1716305?dopt=A

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Specificity:

  The antibody C-11 reacts with cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are members of intermediate filaments subfamily intracellular proteins represented in epithelial tissues.

• Applications Notes:

  Flow cytometry: Recommended dilution: 1 μg/ml. Intracellular staining.Immunohistochemistry: Recommended dilution: 2-8 μg/ml.Western blotting: Recommended dilution: 1-2 μg/ml.

• Isotype:

  Mouse IgG1