Anti-Hu CD11a Purified Azide Free

• Description:

  CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.

• Certification:

  RUO

• Immunogen:

  Human peripheral blood lymphocytes

• Target Antigen:

  CD11a

• Clone:

  MEM-83

• Applications:

  FUNC, FC (QC tested), IP

• Concentration:

  1 mg/mL

• Format:

  Purified Azide Free

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4

• References & Citations:

  *Landis RC, McDowall A, Holness CL, Littler AJ, Simmons DL, Hogg N: Involvement of the "I" domain of LFA-1 in selective binding to ligands ICAM-1 and ICAM-3. J Cell Biol. 1994 Jul;126 (2) :529-37., URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7518468&query_hl=2 , *Landis RC, Bennett RI, Hogg N: A novel LFA-1 activation epitope maps to the I domain. J Cell Biol. 1993 Mar;120 (6) :1519-27., URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7518468&query_hl=2, *Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989) ., URL:

• Other References:

  *Sarantos MR, Raychaudhuri S, Lum AF, Staunton DE, Simon SI: Leukocyte function-associated antigen 1-mediated adhesion stability is dynamically regulated through affinity and valency during bond formation with intercellular adhesion molecule-1. J Biol Chem. 2005 Aug 5;280 (31) :28290-8., URL: https://pubmed.ncbi.nlm.nih.gov/15955822/, *Giblin PA, Lemieux RM: LFA-1 as a key regulator of immune function: approaches toward the development of LFA-1-based therapeutics. Curr Pharm Des. 2006;12 (22) :2771-95., URL: https://pubmed.ncbi.nlm.nih.gov/16918410/, *Kellersch B, Kolanus W: Membrane-proximal signaling events in beta-2 integrin activation. Results Probl Cell Differ. 2006;43:245-57., URL: https://pubmed.ncbi.nlm.nih.gov/17068975/, *Fujii T, Takada-Takatori Y, Kawashima K: Roles played by lymphocyte function-associated antigen-1 in the regulation of lymphocytic cholinergic activity. Life Sci. 2007 May 30;80 (24-25) :2320-4., URL: https://pubmed.ncbi.nlm.nih.gov/17289088/

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Specificity:

  The antibody MEM-83 reacts with an extracellular epitope of CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.

• Species:

  Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG1