Anti-c-myc Antibody

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Anti-c-myc Antibody

CAT:

14-18824-1

Size:

1 mg

Price:

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Anti-c-myc Antibody

Description: Goat Polyclonal Antibody specific to c-myc
Alternative Name: Class E basic helix-loop-helix protein 39, bHLHe39, Proto-oncogene c-Myc, Transcription factor p64
Gene Name: MYC
NCBI Gene ID: MYC
UniProt: P01106
Cellular Locus: Nucleus, nucleoplasm, Nucleus, nucleolus
Host: Goat
Reactivity: C-Myc
Immunogen: Amino acids 410-419 (EQKLISEEDL) of human myc conjugated to KLH.
Target Antigen: Myc proto-oncogene protein
Target: c-myc
Clonality: Polyclonal
Isotype: Whole IgG
Type: Antibody
Applications: WB, IP, ICC, ELISA
Field of Research: Epitope Tags
Purification Method: Purified by peptide immuno-affinity chromatography
Concentration: 1.0 mg/mL
Dilution: Dilute in PBS or medium that is identical to that used in the assay system.
Format: Purified
Form: Liquid
Buffer: Phosphate Buffered Saline
Function: Transcription factor that binds DNA in a non-specific manner, yet also specifically rUniProtKB:P01108, PubMed:20010808, PubMed:24940000, PubMed:25956029}.
Additionnal Information: Immunoblotting: use at a dilution of 1:1,000-1:20,000.Detection of c-myc-tagged protein in 200, 100, and 50ng of E. coli lysate containing tagged fusion protein with #18824 diluted 1:25,000.

Immunocytochemistry: use at a dilution of 1:100-1:400.

ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100- 1:500.

Immunoprecipitation: use at 1-4ug/mg lysate

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
Storage Conditions: This antibody is stable for at least one (1) year at 2-8°C.
Specificity: Antibody was affinity-purified on the peptide.
Formulation: Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Buffer pH: pH 7.2
Target Background: c-myc is an epitope tag often constructed on the N- or C-terminus of a protein to allow for detection and analysis of the protein. The peptide sequence of the myc tag is EQKLISEEDL (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu). The c-myc tag is approximately 1202 Daltons in atomic mass and includes 10 amino acids.

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