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CD4 Rat Monoclonal Antibody (APC)

CAT: 0013-GTR18155292-01Size: 25 assayDry Ice: NoHazardous: No
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CAT#:0013-GTR18155292-01Size:25 assay
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The CD4 Antibody-APC labeled is suitable for FC, IF. It is a Monoclonal, APC conjugated antibody which raised against Native purified mouse CD4.. Purification: The antibody was purified by affinity chromatography.
Product Name Alternative
T-cell surface glycoprotein CD4; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; CD antigen CD4
UniProt
P06332
Reactivity
Mouse
Immunogen
Native purified mouse CD4.
Target
CD4
Clonality
Monoclonal
Clone
BIM3X7
Conjugation
APC
Field of Research
Immunology & Inflammation
Purification
The antibody was purified by affinity chromatography.
Dilution
10 μl / assay
Form
Liquid
Storage Conditions
Shipped and store at 4°C for one year. Do not freeze.
Notes
For research use only.
Applications Notes
1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured) .
Tested Applications
FC, IF
Host or Source
Rat
Preservative
PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
Isotype
Rat IgG2b
Entrez
12504

UniProtKB · P06332

T-cell surface glycoprotein CD4

CD4_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P06332
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Cd4
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

Adaptive immunityImmunity

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembrane

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoproteinLipoproteinPalmitatePhosphoprotein

Domain

Immunoglobulin domainRepeatSignalTransmembraneTransmembrane helix

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