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CD41 Mouse Monoclonal Antibody

CAT: 0013-GTR18155013-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18155013-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The CD41 Antibody is suitable for FC, IF. It is a Monoclonal, Unconjugated antibody .Purification: The antibody was purified by affinity chromatography.
Product Name Alternative
GP2B; ITGAB; Integrin alpha-IIb; GPalpha IIb; GPIIb; Platelet membrane glycoprotein IIb; CD41
UniProt
P08514
Reactivity
Human
Target
ITGA2B
Clonality
Monoclonal
Clone
BR478X3
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
The antibody was purified by affinity chromatography.
Dilution
IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured) .
Tested Applications
FC, IF
Host or Source
Mouse
Preservative
PBS, pH 7.3, and 0.02% sodium azide.
Isotype
Mouse IgG1 kappa
Entrez
3674

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