Products for Research Use Only

Gap43 Antibody

CAT: 0013-GTR18145041Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18145041Size:0.1 mL
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Description
Gap43 Antibody
UniProt
P07936
Reactivity
Human, Mouse, Rat
Immunogen
GAP-43 (Ser41) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser41 of rat GAP-43.
Target
Gap43
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Affinity Purified
Concentration
Batch dependent
Form
Liquid
Molecular Weight
50
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
In some tissues the antibody also recognizes a higher molecular weight band that is also recognized by the Pan GAP-43 antibody and this band may be a GAP-43 aggregate or oligomer. Immunolabeling of the GAP-43 protein in Western blots of rat brain is blocked by the Ser41 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. Note: the GAP-43 protein migrates on SDS-PAGE as a 50 kDa band. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot blots (DB) and Western blots (WB) . Immunohistochemistry (IHC) application has not yet been determined. Human, mouse and rat have 100% amino acid sequence identity with for the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Tested Applications
WB
NCBI Accession Number
P07936
Host or Source
Rabbit

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