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Marcks Antibody

CAT: 0013-GTR18145037Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18145037Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Marcks Antibody
UniProt
P30009
Reactivity
Frog, Human, Mouse, Rat
Immunogen
MARCKS (Ser152/156) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser152 and phospho-Ser156 of rat MARCKS.
Target
Marcks
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Affinity Purified
Concentration
Batch dependent
Form
Liquid
Molecular Weight
87
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Immunolabeling of the 87kDa band in rat brain homogenate is blocked by the Ser152/156 phosphopeptide but not by the non-phosphopeptide. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot Blots (DB) and Western Blot (WB) . Suitability for Immunohistochemistry (IHC) not yet determined. Human, mouse, rat, and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Tested Applications
WB
NCBI Accession Number
P30009
Host or Source
Rabbit

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