Ccl22 Antibody

Ccl22 Antibody

MDC, DCBCK, ABCD-1, Scya22, Abcd1, C-C motif chemokine 22, Activated B and dendritic cell-derived

O88430

Mouse

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMDC. Murine MDC specific antibody was purified by affinity chromatography employing immobilized mMDC matrix.

Ccl22

Unconjugated

Immunology & Inflammation

Batch dependent

Lyophilized

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

For research use only.

Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of mMDC (100 ng/mL), a concentration of 6-9 μg/mL of this antibody is required. ELISA:Indirect:To detect mMDC by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMDC.SandwichTo detect mMDC by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine MDC as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMDC. Western Blot:To detect mMDC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMDC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

ELISA, NeA, WB

O88430