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Ccl4 Antibody

CAT: 0013-GTR18144360Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18144360Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Ccl4 Antibody
Product Name Alternative
Act-2, Mip1b, Scya4, MIP-1B, AT744.1, C-C motif chemokine 4, Immune activation protein 2, ACT-2
UniProt
P14097
Reactivity
Mouse
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMIP-1β. Murine MIP-1β specific antibody was purified by affinity chromatography employing immobilized mMIP-1β matrix.
Target
Ccl4
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Murine MIP-1β (100.0 ng/mL), a concentration of 8.0-10.0 μg/mL of this antibody is required.ELISA:Indirect:To detect mMIP-1b by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-1b.SandwichTo detect mMIP-1b by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine MIP-1b as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-1b. Western Blot:To detect mMIP-1b by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-1b is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Applications
ELISA, NeA, WB
NCBI Accession Number
P14097
Host or Source
Rabbit

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