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APOE Antibody

CAT: 0013-GTR18144343Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18144343Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
APOE Antibody
Product Name Alternative
AD2, LPG, LDLCQ5Apolipoprotein EApo-E
UniProt
P02649
Reactivity
Human
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hApoE3. Human ApoE3 specific antibody was purified by affinity chromatography employing immobilized hApoE3 matrix. *Cross Reactivity: human ApoE2, human ApoE4 in both western blot and sandwich ELISA
Target
APOE
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Metabolism Research
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA:Indirect:To detect hApoE3 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hApoE3.SandwichTo detect hApoE3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human ApoE3 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hApoE3. Western Blot:To detect hApoE3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hApoE3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Applications
ELISA, WB
NCBI Accession Number
P02649
Host or Source
Rabbit

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