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LGALS3 Antibody

CAT: 0013-GTR18144332Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18144332Size:0.1 mg
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Description
LGALS3 Antibody
Product Name Alternative
L31, GAL3, MAC2, CBP35, GALBP, GALIG, LGALS2, Galectin-3, 35 kDa lectin, Gal-3
UniProt
P17931
Reactivity
Human
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Galectin-3. Human Galectin-3 specific antibody was purified by affinity chromatography employing immobilized Galectin-3 matrix.
Target
LGALS3
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Sandwich ELISA:To detect Galectin-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Galectin-3 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Galectin-3. Western Blot:To detect Galectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Galectin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry:This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 31.25 ng/mL with an overnight incubation at 4 ̊C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a pH 6.0 sodium citrate buffer. Optimal concentrations and conditions may vary.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
P17931
Host or Source
Rabbit

UniProtKB · P17931

Galectin-3

LEG3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P17931
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
LGALS3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
Gal-3
EC number
—
Processing
—
Secondary accessions
B2RC38, Q16005, Q6IBA7, Q96J47
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationDisulfide bondPhosphoprotein

Cellular component

CytoplasmNucleusSecretedSpliceosome

Biological process

DifferentiationImmunityInnate immunitymRNA processingmRNA splicing

Molecular function

IgE-binding protein

Ligand

Lectin

Domain

Repeat

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