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WNT1 Antibody (Biotin)

CAT: 0013-GTR18144326Size: 0.05 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18144326Size:0.05 mg
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Description
WNT1 Antibody (Biotin)
Product Name Alternative
INT1, OI15, BMND16, INT1, Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog
UniProt
P04628
Reactivity
Human
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hWNT-1. Human WNT-1 specific antibody was purified by affinity chromatography and then biotinylated.
Target
WNT1
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Stem Cell & Developmental Biology
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
WNT-1 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA:Sandwich:To detect hWNT-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human WNT-1 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-1. Western Blot:To detect hWNT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Applications
ELISA, WB
NCBI Accession Number
P04628
Host or Source
Rabbit

UniProtKB · P04628

Proto-oncogene Wnt-1

WNT1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P04628
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
WNT1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q5U0N2
Protein keywords

Molecular function

Developmental protein

Disease

Disease variantOsteogenesis imperfectaOsteoporosisProto-oncogene

PTM

Disulfide bondGlycoproteinLipoprotein

Cellular component

Extracellular matrixSecreted

Technical term

Proteomics identificationReference proteome

Domain

Signal

Biological process

Wnt signaling pathway

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