Products for Research Use Only

WNT3A Antibody

CAT: 0013-GTR18144325Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18144325Size:0.1 mg
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Description
WNT3A Antibody
Product Name Alternative
Protein Wnt-3a
UniProt
P56704
Reactivity
Human
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hWNT-3a. Human WNT-3a specific antibody was purified by affinity chromatography employing immobilized hWNT-3a matrix.
Target
WNT3A
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA:Indirect:To detect hWNT-3a by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a.SandwichTo detect hWNT-3a by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human WNT-3a as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a. Western Blot:To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Applications
ELISA, WB
NCBI Accession Number
P56704
Host or Source
Rabbit

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