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Egf Antibody (Biotin)

CAT: 0013-GTR18144286Size: 0.05 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18144286Size:0.05 mg
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Description
Egf Antibody (Biotin)
Product Name Alternative
Pro-epidermal growth factorEGF
UniProt
P07522
Reactivity
Rat
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat EGF (Rat Epidermal Growth Factor) . Rat EGF specific antibody was purified by affinity chromatography and then biotinylated.
Target
Egf
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Cancer Biology
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA:Sandwich:To detect Rat EGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Rat EGF as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF. Western Blot:To detect Rat EGF by Western Blot analysis this antibody can be used at a concentration of 0.5 - 1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat EGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Applications
ELISA, WB
NCBI Accession Number
P07522
Host or Source
Rabbit

UniProtKB · P07522

Pro-epidermal growth factor

EGF_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P07522
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Egf
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
EGF
EC number
—
Processing
Precursor
Secondary accessions
Q63183
Protein keywords

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoprotein

Domain

EGF-like domainRepeatSignalTransmembraneTransmembrane helix

Molecular function

Growth factor

Cellular component

Membrane

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