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ICAM1 Antibody

CAT: 0013-GTR18144281Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18144281Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ICAM1 Antibody
Product Name Alternative
BB2, CD54, P3.58Intercellular adhesion molecule 1, Major group rhinovirus receptor, ICAM-1
UniProt
P05362
Reactivity
Human
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTIMP-1 (human Tissue Inhibitor of Metalloproteinases-1) . Human TIMP-1 specific antibody was purified by affinity chromatography and then biotinylated.
Target
ICAM1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human ICAM-1 (10 ng/mL), a concentration of 8.0 μ/mL of this antibody is required.ELISA:Direct:To detect hTIMP-1 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTIMP-1.SandwichTo detect hTIMP-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human TIMP-1 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTIMP-1. Western Blot:To detect hTIMP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTIMP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Applications
ELISA, NeA, WB
NCBI Accession Number
P05362
Host or Source
Rabbit

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