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IL7 Antibody

CAT: 0013-GTR18143834Size: 0.5 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18143834Size:0.5 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
IL7 Antibody
Product Name Alternative
Interleukin 7Interleukin-7IL-7, IL-7
UniProt
P13232
Reactivity
Human
Immunogen
The monoclonal antibody was produced in BALB/c x C57BL/6 F1 mice using recombinant hIL-7 as the immunizing antigen.
Target
IL7
Clonality
Monoclonal
Clone
NYR-hi7
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
This IgG1 antibody was purified from ascites fluid on a Protein A affinity column.
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL 7 (0.5 ng/mL), a concentration of 0.05-0.08 μg/mL of this antibody is required. ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human IL-7 when used with our biotinylated antigen affinity purified anti-human IL-7 (XP-5197Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.Western Blot:To detect hIL-7 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-7 is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
Tested Applications
ELISA, NeA, WB
NCBI Accession Number
P13232
Host or Source
Mouse

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