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TNFSF10 Antibody

CAT: 0013-GTR18143817Size: 0.5 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18143817Size:0.5 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
TNFSF10 Antibody
Product Name Alternative
APO2L, TRAIL, Tumor necrosis factor ligand superfamily member 10, Apo-2 ligand, Apo-2L, TL2, CD253
UniProt
P50591
Reactivity
Human
Immunogen
The monoclonal antibody was produced from a hybridoma using purified recombinant, insect cell derived, hTGFβ1.
Target
TNFSF10
Clonality
Monoclonal
Clone
M66-4B
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
This IgG1k antibody was purified on a Protein G affinity column.
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human TGFβ1 when used in conjunction with compatible secondary reagents. Western Blot:To detect hTGFβ1 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTGFβ1 is 2.0-4.0 ng/lane, under non-reducing conditions. In a dot blot, a 1:500 dilution of this antibody exhibited complete cross reactivity with human TGFβ2 and TGFβ3 isotypes. Reduced cross reactivity was shown at a 1:1000 dilution.
Tested Applications
ELISA, WB
NCBI Accession Number
P50591
Host or Source
Mouse

UniProtKB · P50591

Tumor necrosis factor ligand superfamily member 10

TNF10_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P50591
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
TNFSF10
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A1Y9B3
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

Apoptosis

Cellular component

Cell membraneMembraneSecreted

Molecular function

Cytokine

Ligand

Metal-bindingZinc

PTM

Phosphoprotein

Domain

Signal-anchorTransmembraneTransmembrane helix

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