Gel Shift Binding Buffer 5X Solution

• Description:

  Gel Shift Binding Buffer 5X_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_

  Catalog number:	B2010103
  Lot number:	Batch Dependent
  Expiration date:	Batch Dependent
  Volume/Weight	4 x 500 uL
  pH	7.5
  Supplied as	5X concentrate
  Appearance	Clear solution
  Applications	Binding buffer for gel electrophoresis mobility shift assay (EMSA)
  Shelf-Life	1 year from date of manufacture
  Storage:	Keep at -20°C
  Keywords:	EMSA Binding buffer, electrophoresis mobility shift assay binding buffer
  Grade	Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.
  References	1: Ream JA, Lewis LK, Lewis KA. Rapid agarose gel electrophoretic mobility shift_x000D_ assay for quantitating protein: RNA interactions. Anal Biochem. 2016 Oct_x000D_ 15;511:36-41.2: Hellman LM, Fried MG. Electrophoretic mobility shift assay (EMSA) for_x000D_ detecting protein-nucleic acid interactions. Nat Protoc. 2007;2(8):1849-61.3: Fillebeen C, Wilkinson N, Pantopoulos K. Electrophoretic mobility shift assay_x000D_ (EMSA) for the study of RNA-protein interactions: the IRE/IRP example. J Vis_x000D_ Exp. 2014 Dec 3;(94):52230._x000D_ _x000D_ 4: Ramaswami S, Hayden MS. Electrophoretic mobility shift assay analysis of NF-_x000D_ κB DNA binding. Methods Mol Biol. 2015;1280:3-13._x000D_ _x000D_ 5: Buratowski S, Chodosh LA. Mobility shift DNA-binding assay using gel_x000D_ electrophoresis. Curr Protoc Pharmacol. 2001 Aug;Chapter 6:Unit6.8._x000D_ _x000D_ 6: Künne T, Westra ER, Brouns SJ. Electrophoretic Mobility Shift Assay of DNA_x000D_ and CRISPR-Cas Ribonucleoprotein Complexes. Methods Mol Biol. 2015;1311:171-84._x000D_ _x000D_ 7: Csorba T, Burgyán J. Gel mobility shift assays for RNA binding viral RNAi_x000D_ suppressors. Methods Mol Biol. 2011;721:245-52._x000D_ _x000D_ 8: Poulin-Laprade D, Burrus V. Electrophoretic Mobility Shift Assay Using_x000D_ Radiolabeled DNA Probes. Methods Mol Biol. 2015;1334:1-15._x000D_ _x000D_ 9: Sadakane Y, Hatanaka Y. Photoaffinity electrophoretic mobility shift assay_x000D_ using photoreactive DNA bearing 3-trifluoromethyl-3-phenyldiazirine in its_x000D_ phosphate backbone. Anal Biochem. 2016 Aug 1;506:1-7._x000D_ _x000D_ 10: Carruthers NJ, Parker GC, Gratsch T, Caruso JA, Stemmer PM. Protein Mobility_x000D_ Shifts Contribute to Gel Electrophoresis Liquid Chromatography Analysis. Version_x000D_ 2. J Biomol Tech. 2015 Sep;26(3):103-12._x000D_ _x000D_ 11: Memelink J. Electrophoretic mobility shift assay for the analysis of_x000D_ interactions of jasmonic acid-responsive transcription factors with DNA. Methods_x000D_ Mol Biol. 2013;1011:209-25._x000D_ _x000D_ 12: García-Solaesa V, Sanz-Lozano CS. Interactions of DNA and Proteins:_x000D_ Electrophoretic Mobility Shift Assay in Asthma. Methods Mol Biol._x000D_ 2016;1434:91-105._x000D_ _x000D_ 13: Morita T, Maki K, Aiba H. Detection of sRNA-mRNA interactions by_x000D_ electrophoretic mobility shift assay. Methods Mol Biol. 2012;905:235-44. d
  Related Products	Biochemical Buffers and Solutions

• Short Description:

  Catalog Number: B2010103 (4 x 500 uL)_x000D_ Gel Shift Binding Buffer 5X is a highly optimized, reproducible, buffer for use in Gel Electrophoresis Mobility Shift Assay (EMSA) and similar applications. Its proprietary composition counteracts non-specific interactions and results in sharp EMSA gel profiles. This product is supplied as a clear solution aliquoted in four vials, 500 uL each. Custom bulk orders are available upon request.

• Weight:

  2

• Length:

  2.5

• Width:

  2

• Height :

  1.2