Rabbit IgG, conjugated with FITC

Rabbit IgG, conjugated with FITC

• Background: As an efficient general counterstain for cell and tissue substrates with immunoconjugates with a label contrasting with the greenish-yellow fluorescence of this product. The purpose of counterstaining is to enhance the optical contrast of the immunospecific staining against a counterstained background.
• Conjugation: FITC
• Assay Principle: ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence. The conjµgate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at -20°C. Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water. Spin down to remove insoluble particles, divide into small aliquots, and store frozen at or below -20°C. Prior to use an aliquot is thawed at ambient temperature, spun down again, and used to prepare working dilutions by adding sterile PBS pH7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifµgation. It will not affect the performance of the conjµgate. Diluted product should be stored at +4°C, not refrozen, and preferably used the same day.
• Form: Fluorochrome coupled purified rabbit IgG in phosphate buffered saline (pH7,2); No preservative added
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.