Lambda Protein Phosphatase (Lambda PP)

• Description:

  Lambda Protein Phosphatase (Lambda PP) is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine and tyrosine residues. One unit is defined as the amount of enzyme that hydrolyzes 1 nmol of p-Nitrophenyl Phosphate (50 mM) in 1 minute at 30°C in a total reaction volume of 50 µl.

• Expression System:

  E. coli

• Nature:

  Recombinant

• Applications:

  Remove phosphate groups from phosphorylated serine, threonine and tyrosine residues in proteins.

• Stability:

  Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.

• Endotoxin:

  Please contact with the lab for this information.

• Purity:

  >90% as determined by SDS-PAGE.

• Form:

  Liquid

• Buffer:

  100 mM NaCl, 50 mM HEPES, pH 7.5, 0.1 mM MnCl2, 0.1 mM EDTA, 2 mM DTT, 0.01% Brij35, and 50% glycerol.

• Shipping Conditions:

  In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

• Notes:

  Avoid repeated freeze/thaw cycles. For research use only.

• Species:

  Escherichia phage lambda (Bacteriophage lambda)