VPS39 antibody

• Background:

  Regulator of TGF-beta/activin signaling, inhibiting SMAD3-and activating SMAD2-dependent transcription. Acts by interfering with SMAD3/SMAD4 complex formation, this would lead to inhibition of SMAD3-dependent transcription and relieve SMAD3 inhibition of SMAD2-dependent promoters, thus increasing SMAD2-dependent transcription. Does not affect TGF-beta-induced SMAD2 or SMAD3 phosphorylation, nor SMAD2/SMAD4 complex formation. Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act in part as a component of the putative HOPS endosomal tethering complex which is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes (PubMed:23351085) . Involved in homotypic vesicle fusions between late endosomes and in heterotypic fusions between late endosomes and lysosomes (PubMed:11448994, PubMed:23351085, PubMed:23167963) . Required for fusion of endosomes and autophagosomes with lysosomes (PubMed:25783203) .

• Synonyms:

  Vam6/Vps39-like protein|TRAP1-like protein (hVam6p) |VPS39|KIAA0770|TLP|VAM6

• Gene ID:

  23339

• UniProt:

  Q96JC1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  Vacuolar protein sorting 39 homolog

• Target:

  VPS39

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, WB

• Field of Research:

  Signal Transduction

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:500 - 1:2000

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  102 kDa

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, WB

• Immunogen Sequence:

  630-886aa