VPS18 antibody

• Background :

  Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed:11382755, PubMed:23351085, PubMed:24554770, PubMed:25783203) . Required for fusion of endosomes and autophagosomes with lysosomes (PubMed:25783203) . Involved in dendrite development of Pukinje cells (By similarity) .

• Synonyms :

  Vacuolar protein sorting-associated protein 18 homolog (hVPS18) |VPS18|KIAA1475

• Gene ID :

  57617

• UniProt :

  Q9P253

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Vacuolar protein sorting 18 homolog

• Target :

  VPS18

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  ELISA, IP, IHC, FC, IF

• Field of Research :

  Signal Transduction

• Purification :

  Immunogen affinity purified

• Dilution :

  WB: 1:200-1:1000; IHC: 1:50-1:500; IP: 1:200-1:1000; IF: 1:20-1:200

• Purity :

  ≥95% as determined by SDS-PAGE

• Form :

  Liquid

• Molecular Weight :

  110 kDa

• Storage Conditions :

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications :

  ELISA, IP, IHC, FC, IF