UBE2I-Specific antibody

• Background:

  UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBE2I is detected in kidney and liver. Low amount of UBE2I is observed in brain, whereas the 18 kDa band of UBE2I is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBE2I antibodies recognizes a 38 kDa protein band, but this band is not visible in extracts of other rat tissues (PMID:14739995) .

• Synonyms:

  SUMO-conjugating enzyme UBC9|RING-type E3 SUMO transferase UBC9|SUMO-protein ligase|Ubiquitin carrier protein 9|Ubiquitin carrier protein I|Ubiquitin-conjugating enzyme E2 I|Ubiquitin-protein ligase I|p18|UBE2I|UBC9|UBCE9

• UniProt:

  P63279

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)

• Target:

  UBE2I

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, WB, IHC, IF, FC

• Field of Research:

  Epigenetics, Metabolism, Developmental biology, Neuroscience, Cell Division and Proliferation

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:10-1:100

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  18 kDa

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, WB, IHC, IF, FC

• Immunogen Sequence:

  Peptide