TOP3A antibody

• Background:

  Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA- (5'-phosphotyrosyl) -enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity.

• Synonyms:

  DNA topoisomerase 3-alpha|DNA topoisomerase III alpha|TOP3A|TOP3

• Gene ID:

  7156

• UniProt:

  Q13472

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Topoisomerase (DNA) III alpha

• Target:

  TOP3A

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, WB, IP

• Field of Research:

  Metabolism

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:500-1:2000; IP: 1:200-1:2000

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  100-110 kDa

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, WB, IP