TAP1 antibody

• Background:

  Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules.

• Synonyms:

  Antigen peptide transporter 1 (APT1) |ATP-binding cassette sub-family B member 2|Peptide supply factor 1|Peptide transporter PSF1 (PSF-1) |Peptide transporter TAP1|Peptide transporter involved in antigen processing 1|Really interesting new gene 4 protein (RING4) |TAP1|ABCB2|PSF1|RING4|Y3

• Gene ID:

  6890

• UniProt:

  Q03518

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  Transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)

• Target:

  TAP1

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, IF, WB, IHC, IP

• Field of Research:

  Immunology

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:500-1:2000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  70-81 kDa

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, IF, WB, IHC, IP