Spast antibody

ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated (PubMed:19141076 PubMed:20530212) . Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (By similarity) . Severing activity is not dependent on tubulin acetylation or detyrosination (By similarity) . Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation (By similarity) . It is critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia (By similarity) . SPAST is involved in abscission step of cytokinesis and nuclear envelope reassembly during anaphase in cooperation with the ESCRT-III complex (By similarity) . Recruited at the midbody, probably by IST1, and participates to membrane fission during abscission together with the ESCRT-III complex (By similarity) . Recruited to the nuclear membrane by IST1 and mediates microtubule severing, promoting nuclear envelope sealing and mitotic spindle disassembly during late anaphase (By similarity) . Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and endosome recycling (By similarity) . Recruited by IST1 to endosomes and regulates early endosomal tubulation and recycling by mediating microtubule severing (By similarity) . Probably plays a role in axon growth and the formation of axonal branches (PubMed:18234839) . Isoform 1: Involved in lipid metabolism by regulating the size and distribution of lipid droplets.

Spastin|Spast|Kiaa1083|Spg4

50850

Q9QYY8

Human, Mouse, Rat

Spastin

Spast

ELISA, WB

Metabolism, Developmental biology

Immunogen affinity purified

WB: 1:500-1:2000

≥95% as determined by SDS-PAGE

Liquid

52 kDa

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)