REST antibody

• Background:

  Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression.

• Synonyms:

  RE1-silencing transcription factor|Neural-restrictive silencer factor|X2 box repressor|REST|NRSF|XBR

• Gene ID:

  5978

• UniProt:

  Q13127

• Host:

  Rabbit

• Reactivity:

  Human, Rat

• Immunogen:

  RE1-silencing transcription factor

• Target:

  REST

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, WB, IP

• Field of Research:

  Stem Cells, Metabolism

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:500-1:1000; IP: 1:500-1:2000

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  122 kDa, 200kd

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, WB, IP