RAB5A antibody

• Background:

  The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032) . Contributes to the regulation of filopodia extension (PubMed:14978216) . Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413) . Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity) .

• Synonyms:

  Ras-related protein Rab-5A|RAB5A|RAB5

• Gene ID:

  5868

• UniProt:

  P20339

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  RAB5A, member RAS oncogene family

• Target:

  RAB5A

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, IHC, WB, IP,IF

• Field of Research:

  Neuroscience, Signal Transduction

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:200-1:2000; IP:1:200-1:2000; IHC: 1:50-1:500; IF: 1:50-1:500

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  24 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, IHC, WB, IP, IF