POLR2F antibody

• Background :

  DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and together with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds (By similarity) .

• Synonyms :

  DNA-directed RNA polymerases I, II, and III subunit RPABC2 (RNA polymerases I, II, and III subunit ABC2) |DNA-directed RNA polymerase II subunit F|DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide|RPABC14.4 (RPB14.4) |RPB6 homolog|RPC15|POLR2F|POLRF

• Gene ID :

  5435

• UniProt :

  P61218

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Polymerase (RNA) II (DNA directed) polypeptide F

• Target :

  POLR2F

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  ELISA, WB, IF, IHC

• Field of Research :

  Epigenetics, Metabolism

• Purification :

  Immunogen affinity purified

• Dilution :

  WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200

• Purity :

  ≥95% as determined by SDS-PAGE

• Form :

  Liquid

• Molecular Weight :

  14 kDa

• Shipping Conditions :

  4°C with ice bag

• Storage Conditions :

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications :

  ELISA, WB, IF, IHC