Phospho-Akt(Ser473) antibody

Phospho-Akt(Ser473) antibody

• Background: The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with phospho-modification.
• Synonyms: RAC-alpha serine/threonine-protein kinase|Protein kinase B (PKB)|Protein kinase B alpha (PKB alpha)|Proto-oncogene c-Akt|RAC-PK-alpha|AKT1|PKB|RAC
• CAS Number: 9007-83-4
• UniProt: P31749
• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Immunogen: v-akt murine thymoma viral oncogene homolog 1
• Target: Phospho-Akt(Ser473)
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 5H4
• Applications: ELISA, IHC, WB
• Field of Research: Metabolism, Cardiovascular, Immunology, Developmental biology, Neuroscience, Stem Cells
• Purification: Protein A+G purification
• Dilution: WB: 1:1000-1:4000; IHC: 1:50-1:200
• Purity: ≥95% as determined by SDS-PAGE
• Form: Liquid
• Molecular Weight: 60 kDa
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze / thaw cycles.)