CD274 antibody

• Background:

  Programmed cell death ligand 1 (CD274, or B7-H1, PD-L1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. CD274 is suggested a negative regulator of T and B cell, and play important role in mediating tolerance of lymphocytes to self-antigens. It also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. PD-L1 is a 290 aa transmembrane protein with a calculated molecular weight of 33 kDa, the apparent molecular weight has been reported as 45-70 kDa, suggesting probable glycosylation (PMID: 18760278; 16493058) .?

• Synonyms:

  Programmed cell death 1 ligand 1 (PD-L1, PDCD1 ligand 1, Programmed death ligand 1, hPD-L1) |B7 homolog 1 (B7-H1) |CD274|B7H1|PDCD1L1|PDCD1LG1|PDL1

• Gene ID:

  29126

• UniProt:

  Q9NZQ7

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  CD274 molecule

• Target:

  CD274

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, WB, IF, IHC, IP, FC

• Field of Research:

  Cancer, Immunology, Signal Transduction

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:200-1:1000; IP: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:10-1:100

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  50 kDa, 70 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, WB, IF, IHC, IP, FC

• Immunogen Sequence:

  25-241 aa