PCNA antibody

• Background:

  Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902) . Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

• Synonyms:

  Proliferating cell nuclear antigen (PCNA) |Cyclin|PCNA

• Gene ID:

  5111

• UniProt:

  P12004

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat, rabbit

• Immunogen:

  Proliferating cell nuclear antigen

• Target:

  PCNA

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Applications:

  ELISA, WB, IHC, FC, IP

• Field of Research:

  Epigenetics, Metabolism, Immunology, Cancer, Cell Division and Proliferation

• Purification:

  Protein A+G purification

• Dilution:

  WB: 1:5000-1:50000; IP: 1:200-1:1000; IHC: 1:500-1:2000; IF: 1:50-1:500

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  38 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Clone ID:

  8F9

• Tested Applications:

  ELISA, WB, IHC, FC, IP