PARD3 antibody

• Background:

  Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions. Involved in Schwann cell peripheral myelination (By similarity) . Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.PARD3 has three splice isoforms at 100 kDa, 150 kDa, and 180 kDa. This polyclonal antibody raised against C-terminal 281 amino acids of human PARD3 recognizes these three isoforms.

• Synonyms:

  Partitioning defective 3 homolog (PAR-3, PARD-3) |Atypical PKC isotype-specific-interacting protein (ASIP) |CTCL tumor antigen se2-5|PAR3-alpha|PARD3|PAR3|PAR3A

• Gene ID:

  56288

• UniProt:

  Q8TEW0

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Partitioning defective 3 homolog

• Target:

  PARD3

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, WB, IHC, FC, IP

• Field of Research:

  Neuroscience

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:500-1:1000; IP: 1:200-1:1000; IHC: 1:50-1:200; IF: 1:50-1:500

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  100 kDa, 150 kDa, and 180 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, WB, IHC, FC, IP

• Immunogen Sequence:

  180-442aa