OSBP antibody

• Background:

  Lipid transporter involved in lipid countertransport between the Golgi complex and membranes of the endoplasmic reticulum: specifically exchanges sterol with phosphatidylinositol 4-phosphate (PI4P), delivering sterol to the Golgi in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum (PubMed:24209621) . Binds cholesterol and a range of oxysterols including 25-hydroxycholesterol (PubMed:15746430, PubMed:17428193) . Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylates ERK1/2, whereas 25-hydroxycholesterol causes its disassembly (PubMed:15746430) . Regulates cholesterol efflux by decreasing ABCA1 stability (PubMed:18450749) .

• Synonyms:

  Oxysterol-binding protein 1|OSBP|OSBP1

• Gene ID:

  5007

• UniProt:

  P22059

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Oxysterol binding protein

• Target:

  OSBP

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, WB, IHC, IP

• Field of Research:

  Cardiovascular, Metabolism

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  89 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, WB, IHC, IP