NT5E antibody

• Background :

  NT5E (5'-nucleotidase) is also named as NT5, NTE, CD73, ecto-5'-nucleotidase, and belongs to the 5'-nucleotidase family. It catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP.The enzyme occurs mainly as a dimer and the wide distribution of NT5E in the rat hippocampus, suggesting their involvement in the control of the purinergic signaling (PMID:17619139) . This protein has two isoforms with the molecular weight of 63 kDa and 58 kDa and four glycosylation sites with a signalpeptide. It can exsit as a dimer (PMID:17619139) . It can be detected the band of 55 kDa,64 kDa, 70 kDa, 110 kDa by western blot (PMID:17619139; 12370277; 21533188) . Defects in NT5E are the cause of calcification of joints and arteries (CALJA) .

• Synonyms :

  5'-nucleotidase (5'-NT) |5'-deoxynucleotidase|Ecto-5'-nucleotidase|IMP-specific 5'-nucleotidase|Thymidylate 5'-phosphatase|NT5E|NT5|NTE

• Gene ID :

  4907

• UniProt :

  P21589

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  5'-nucleotidase, ecto (CD73)

• Target :

  NT5E

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  ELISA, WB,IHC,FC

• Field of Research :

  Metabolism, Cardiovascular, Immunology, Cancer, Stem Cells

• Purification :

  Immunogen affinity purified

• Dilution :

  WB: 1:500-1:5000; IHC: 1:50-1:500

• Purity :

  ≥95% as determined by SDS-PAGE

• Form :

  Liquid

• Molecular Weight :

  63 kDa

• Shipping Conditions :

  4°C with ice bag

• Storage Conditions :

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications :

  ELISA, WB, IHC, FC