NES antibody

• Background:

  Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.

• Synonyms:

  Nestin|NES

• Gene ID:

  10763

• UniProt:

  P48681

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Nestin

• Target:

  NES

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Applications:

  ELISA, WB, IHC, IF

• Field of Research:

  Cardiovascular, Immunology, Cancer, Developmental biology, Neuroscience, Stem Cells

• Purification:

  Protein A+G purification

• Dilution:

  WB: 1:500-1:2000; IHC: 1:20-1:1000; IF: 1:20-1:200

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  170 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Clone ID:

  4F9

• Tested Applications:

  ELISA, WB, IHC, IF